Coding

Part:BBa_K640000:Design

Designed by: Emily Hicks   Group: iGEM11_Calgary   (2011-09-25)

Codon optimized Luciferase for use in microalgae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 547


Design Notes

This part was originally received from the chlamydomonas database (chlamydb.org). This part contained no illegal cut sites and the Biobrick prefix and suffix were added via polymerase chain reaction.


Source

http://www.chlamy.org/chlamydb.html

References

Fuhrmann et al. (2004) 

  Plant Mol. Biol. 55, 869-881

Heitzer and Zschoernig (2007) 

  Biotechniques 43, 324